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This can result in: Reduced pain Enhanced joint stability Improved range of motion Quicker return to normal activity Ideal Candidates for Prolotherapy Prolotherapy is most effective in Type I to Type III AC separations , especially when: Pain persists beyond 4-6 weeks of conservative care There is evidence of ligament laxity or instability The patient prefers a non-surgical option The patient is physically active and motivated for recovery The Prolotherapy Treatment Protocol for AC Separation Pre-Treatment Evaluation Prior to treatment, a comprehensive musculoskeletal exam is conducted to assess: Joint instability Tenderness and ligament integrity Shoulder biomechanics Imaging (X-ray or ultrasound) to classify injury severity Injection Technique Patient Positioning: Seated or supine with shoulder exposed Site Preparation: Skin cleaned with antiseptic

This slow increase helps reduce gastrointestinal side effects and improves overall tolerability

The table below shows example preparations vial size, an example water volume, and the resulting concentration

TRAb/TBII can be measured with enzyme linked immunosorbent assays (ELISA) that measure the ability of the patients serum antibodies to inhibit binding of labeled TSH-R ligands (human monoclonal thyroid antibodies) to the TSH-R (209)