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The following antibodies were used: antibodies against human eNOS, phosphor-eNOS (Ser1177), Cav-1, phosphor-Cav-1 (Tyr14), Src and phosphor-Src (Tyr416) were obtained from Cell Signaling (MA, USA)

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We identified 48 protein spots in 2-D gel analysis and selected proteins were divided into 64% collagen components and 30% non-collagen components as shown by the MALDI-TOF analysis

Published research protocols use administration frequencies ranging from daily to three-times-weekly depending on study objectives, with intermittent dosing often producing comparable or superior results to daily administration in long-term neuroplasticity studies