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GlycoATP and mitoATP were derived from cells sequentially treated with oligomycin A and Rot/Antimycin A

We therefore suggest that our findings are valid representations of the relativities of each parameter among strains, temperatures and time-points, and their various interactions, whilst also recognizing the additional value in calibrating our methodology against absolute quantifications of the underlying biochemistries in future studies

Cells were washed and incubated with Cy-3 or Cy-5 conjugated secondary antibodies (Jackson ImmunoResearch Laboratories, West Grove, PA and Thermofisher)

The cells were then washed with PBS buffer, and 1 10^6 cells were taken for each sample

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