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Thus our assay allows us to separate and identify M

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Subsequently, all samples were subjected to centrifugation at 1500 rpm for 15 min at 4 C, facilitating a clear delineation of the two phases: the upper (lipophilic) phase, which contains the apolar metabolites, and the lower (hydrophilic) phase, which comprises the polar metabolites of interest

Hear Res 46:113145 Suvarna KS, Layton C, Bancroft JD (2018) Bancroft's theory and practice of histological techniques E-book