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Based on mechanism, two interaction concerns are worth tracking: Additive collagen synthesis

It inhibits the pro-survival Akt and ERK1/2 signaling pathways, thereby reducing proliferation and resistance to apoptosis, while also enhancing p53 stability and transcriptional activity, restoring its tumor-suppressive role and promoting hormonally responsive decidualization, a physiological process whose impairment contributes to EMs implantation and persistence (Yu J

After buffer addition, samples were vortexed to ensure complete lysis and then incubated in the dark at room temperature for 20 min to allow for the alkylation of exposed cysteine residues