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Beyotime Institute of Biotechnology) was performed at room temperature for 30 min, following by primary antibody addition at 4C overnight

147 As a small molecule Nrf2 activator, sulforaphane increased the gene expression of Nrf2, reduced the level of ROS, and prevented the damage of CSE-treated alveolar epithelial cells

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Circulating GSH levels were also significantly decreased (Supplemental Figure 1D), indicating liver is the primary organ supplying GSH to the circulation