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Glucose and insulin tolerance tests Two-month-old mice that were fed with a 60% calorie-rich HFD for 120 days were fasted for 6 hours before glucose and insulin tolerance tests, Blood was collected from these mice by tail nicking to measure glucose concentrations

Ethics declarations Authors note These results were presented in parts at the Metabolomics in Life Science conference, 2728 January 2026, Ume, Sweden

[82] The double-stranded RNAs, through either the small interfering RNA (siRNA) or microRNA (miRNA) pathways direct de-novo DNA methylation of the original genomic location that produced the RNA