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J., Burns, W., Hayashi, K., Geis, S

To generate sgRNA-resistant STK38, site-directed mutagenesis was performed on pCDH-hygro/STK38 using the following primers: Forward: 5-TTTTATAGCAACTTGATAGCCCAGCATG-3, Reverse: 5-CATGCTGGGCTATCAAGTTGCTATAAAA-3

For gradual thermal denaturation, TgCBS was incubated at temperatures between 30 and 70 C for 10 min, cooled on ice for 5 min, and then residual enzymatic activity toward l-Ser was assayed in buffer containing 0.2 mM NADH, 2 M LDH, 1.5 M CBL, 0.8 mM l-Hcys, and 10 mM l-Ser

Studies show that the production of lactic acid is increased by a factor of eight in this situation, with no change in oxygen levels

These reports are encouraging