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Before human translation, essential parameters to define: optimal dosing, infusion/administration route, frequency, biomarkers to monitor (senescent cell burden, inflammatory cytokines, organ function), long-term follow-up for adverse outcomes (especially tumour risk)

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Two degradation pathways matter: Hydrolysis: Water cleaves peptide bonds, particularly at aspartate-proline sequences, which are among the most hydrolysis-prone linkages in peptide chemistry

Finally, the remodeling phase reorganizes this new tissue into functional architecture that can withstand mechanical stress