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This may be too high because Weger (1968) observed severe circulatory collapse in volunteers after 12 mg/kg was given intravenously

Munker R, Younes A, Cabanillas F, Andreeff M

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In samples containing analyte, nonlabeled IgG will compete with Tracer-LgBiT for binding to the FcR target, resulting in a concentration-dependent decrease in the luminescent signal