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Following title and abstract screening for relevance to digital technologies and the diseases of interest (Fig

Many NGP candidates are significant contributors to butyrate and propionate pools, thereby supplying energy substrates for colonocytes, enhancing epithelial barrier function, and exerting epigenetic and anti-inflammatory effects through HDAC inhibition and G-proteincoupled receptor signaling (96)

The TRIUMPH-4 trial showed that retatrutide at 12mg reduced: Systolic blood pressure: 14.0 mmHg reduction Non-HDL cholesterol: clinically meaningful improvement High-sensitivity C-reactive protein: significant reduction (a marker of systemic inflammation) Triglycerides: substantial decrease Tirzepatide produces similar cardiovascular improvements, with well-documented reductions in blood pressure, triglycerides, and inflammatory markers across the SURMOUNT and SURPASS programs

However, motor function was not evaluated in this prior study

For antibody staining, antigen retrieval was performed on the slides by incubating them in a steamer for 40 min in citrate buffer (Vector Labs, Cat# H-3300-250)