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Proline, as a non-proteinogenic amino acid, is well-known for its roles in plant adaptation to abiotic stresses, including heat stress, in various plant species and functions during osmotic adjustment for cellular turgor maintenance, the maintenance of membrane stability, and antioxidant protection (Hayat et al

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Residual sugars in fermented ciders were measured using a Megazyme (Bray, Ireland) Sucrose, D-Fructose, and D-Glucose kit in a 96-well microplate spectrophotometric method at 340 nm