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The clarified cell lysate was either directly submitted to further protein purification steps or flash-frozen in liquid nitrogen after addition of 10% glycerol and stored at 80 C for use in biocatalytic reactions

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Earlier secretagogues such as GHRP-6 and GHRP-2 do trigger GH release, but they also switch on other pathways: they can raise cortisol (a stress hormone), raise prolactin, and drive a strong hunger response