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Protective effects of restricted diet and antioxidants on testis tissue in rats fed with high-fat diet

The rash of POD is bumpy and scaly in appearance

To evaluate the mitochondrial membrane potential by flow cytometry, the cells were therefore detached by using trypLE as described in 2.1, re-suspended the cells in complete cell culture medium (1 10 6 /ml) and treated with rotenone (0.5 M, 2 h) in an eppendorph tube