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Quantification of H 2 S and GSSH from oxidatively stressed mitochondria Rat heart mitochondria (1 mg protein/mL) were suspended in KCl buffer (pH 7.2) containing 1 mM EGTA and treated with carrier (KCl buffer), Na 2 S (250 M) or H 2 O 2 (50 M) for 3 min, or H 2 O 2 (50 M) for 3 min followed by and Na 2 S (250 M) for 3 min at RT

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Cumulative incidence of outcomes was determined by the Kaplan-Meier method

Wang et al., 2022

The percentage of on-target recombination including insertions and deletions (indels) in the genomic DNA from these cell populations was evaluated by Sanger sequencing followed by analysis using the Tide web-based tool ( The sequences of gRNAs used are provided below: Electroporation of 3T3-L1 Mlkl -KO cells Electroporation was performed using the Amaxa Nucleofector 2b system (VCA-1003