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Cell culture HeLa cells were grown in DMEM supplemented with fetal bovine serum (FBS, 10%) and penicillin (100 U ml 1 )/streptomycin (100 g ml 1 )/fungizone (0.25 g ml 1 ) at 37 C in a 5% CO 2 environment

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It is characterized as a multifactorial condition that encompasses tear film instability, ocular surface damage, inflammation, hyperosmolarity, and neurosensory abnormalities [62]