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Silvagno et al., 2020)

Proc Natl Acad Sci USA 107(16):74557460 Ippolito L, Giannoni E, Chiarugi P, Parri M (2020) Mitochondrial redox hubs as promising targets for anticancer therapy

Douglas J Lanska MD MS MSPH Dr

After derivatization with 10 l of 5 mM 7-diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin (Sigma-Aldrich) at room temperature for 10 min, the maleimide ring was cleaved by addition of 30 l of 50 mM ammonium formate (Sigma-Aldrich) pH 4.2 at room temperature for another 30 min