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[Abstract] Immunoblotting with anti-GST antibodies was used to detect the binding of recombinant His-ANT2 to GST-GRP75

Sigma-Aldrich, Germany) and mixed for another 90 min at 37C

To investigate the impact of shifting from cystine-driven to GSH-driven cysteine supply in cancer cells in an unbiased manner, we performed a multifunctional approach to pharmacological screening (MAPS) with a library of 240 metabolic inhibitors (Fig