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Grape-derived EVs in oral administration were used in the prevention and treatment of oral mucositis associated with radiotherapy for head and neck cancer (NCT01668849)

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After washing in PBS they were resuspended in 200 l Guava Cell Cycle Reagent (containing PI and other dyes) and after 30 min at room temperature in the dark loaded onto the Guava 8HT to differentiate cells according to DNA content (G0/G1, S and G2/M)

RNA isolation and real-time quantitative reverse-transcriptase PCR (qRT-PCR) analysis For RNA extraction, the peqGold RNA isolation kit (Peqlab, Erlangen, Germany) was used for phLF and the RNeasy Mini Plus Kit (Quiagen

2007), in addition to ABCB1/Pgp protein stabilization through heat shock protein 90 (Bertram et al