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Multivariate Cox proportional hazards method was used for analyzing the relationship between the variables and survival time

2022 Apr 12;39(2):110660

In the experiments, ATP-triggered CPT release showed significant anticancer effects on MDA-231 breast cancer cells, with a cell death rate of 65% compared to 25% in normal breast cells, indicating that this system is able to discriminate between cancerous and normal cells

To eliminate technical variability during acquisition, individual samples within one experiment were fixed with Maxpar Fix I Buffer for 10 min at RT, permeabilized with Maxpar Barcode Perm Buffer, and barcoded with the Cell-ID palladium (Pd) barcoding kit (Fluidigm) for 45 min at RT