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There are five locations for in ovo delivery in chickens, namely, the air cell, embryo, yolk sac, allantoic membrane, and amniotic fluid ( in ovo technology was adapted over time for in ovo feeding of probiotics or prebiotics on embryonic day (e)18 or synbiotics delivery on e12 ( in ovo delivery on e12 is referred to as in ovo stimulation, whereas delivery on e1718 is known as in ovo feeding ( in ovo delivery for feeding purposes is late-term embryo and the amniotic fluid, respectively ( in ovo procedure on e12 involves delivery of bioactive compounds into the egg's air cell

Primer sequences are shown in (Table 1)

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Wang J, Tang W, Yang M, Yin Y, Li H, Hu F, Tang L, Ma X, Zhang Y, Wang Y

Baldominos, P