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2.8 Spectroscopic MSA-DH or MDH activity assays The activity of MSA-DH or MDH was measured in a continuous spectroscopic assay by determining the initial rate of NADH formation at 340 nm, at a temperature of 27C using an extinction coefficient of 340 = 6.2 mM 1 cm 1 (Hossain et al., 1984)

Food Chem 129:345350 Garzarella EU, Navajas-Porras B, Prez-Burillo S et al (2022) Evaluating the effects of a standardized polyphenol mixture extracted from poplar-type propolis on healthy and diseased human gut microbiota