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Generation of Fluorescent Molecules through Chemical Reaction or Enzymatic Cascade Reaction Compared with ascorbic acid 2-phosphate (AAP), the enzymatic product ascorbic acid (AA) in the dehydrogenized format can quickly react with o -phenylenediamine (OPD) to form N -heterocyclic fluorophore under alkaline conditions (Scheme 2) [65,66]

The authors gratefully acknowledge the following veterinarians and veterinary technicians for their assistance with Kristen Antoon, Amelie Beaumier, Melanie Borglund, Sara Brethel, Suzanne Cunningham, Luis Dos Santos, Lana Fagman, Renee Girens, Courtney Hanner, Viviana Heinekin, Emily Karlin, Katherine Lopez, Michelle Maillet, Melissa Pisaroglo de Carvalho, Camden Rouben, Michelle Vereb, and Vicky Yang, with a special thanks to Kelsey Weeks, CVT, RVT g

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