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Transfection of cells, Immunofluorescence microscopy and Subcellular fractionation of transfected cells For subcellular fractionation, HEK293T cells originating from ATCC (CRL-3216) were transfected using Ca-phosphate precipitation method

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Annu Rev Plant Biol 59:143166 Sappl PG, Carroll AJ, Clifton R, Lister R, Whelan J, Millar AH, Singh KB (2009) The Arabidopsis glutathione transferase gene family displays complex stress regulation and co-silencing of multiple genes results in altered metabolic sensitivity to oxidative stress