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A study by Zylka et al

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Long R3 IGF-1, Long Arginine 3-IGF-1, LR3-IGF-1 20-30 hours (vs 12-15 minutes for native IGF-1) IGF-1 LR3 (Long R3 IGF-1) is a modified insulin-like growth factor 1 with an arginine substitution at position 3 and a 13-amino acid N-terminal extension

Tf was subsequently conjugated to the nanoparticle surface via a carbodiimide coupling reaction