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After euthanasia, the pancreas was perfused via the common bile duct with 24 mL of digestion buffer (Hanks Balanced Salt Solution containing type IV collagenase, 1 mg/mL, Gibco, 17104019)

Trunk blood samples were drawn and liver and kidney tissue samples were taken on day 10

"Diagnostic accuracy of the Amsler grid and the preferential hyperacuity perimetry in the screening of patients with age-related macular degeneration: systematic review and meta-analysis"