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Statements Author contributions XD: Conceptualization, Writing original draft, Writing review and editing, Validation, Visualization

Common Symptoms of B12 Deficiency The signs of deficiency can vary but often include: Chronic fatigue Tiredness that doesnt improve with rest Brain fog Poor memory, lack of concentration, confusion Mood changes Depression, irritability, or anxiety Numbness or tingling Especially in hands and feet Pale or yellowed skin Muscle weakness Balance issues or dizziness Shortness of breath In severe cases, untreated deficiency can lead to irreversible nerve damage and anemia

Once outside the cell, GSH degradation occurs exclusively in the extracellular space and only on the surface of cells that express the ectoenzyme -glutamyl transferase

Healthy digestion starts with strong stomach acid

Analytical Techniques Gas ChromatographyMass Spectrometry The first step was the derivatization of glutathione and analysis of N , S -ethoxycarbonyl methyl esters (NSECM) by the GCMS method