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The rationale for why hypertonic saline could augment loop diuretics is as follows: Hypertonic saline could improve chloride levels, thereby reversing the effects of hypochloremia on sodium retention (explored above)

Researchers must dissolve the compound via a slow, gentle swirling motion, avoiding any aggressive mechanical agitation, inversion, or shaking, which can permanently disrupt the cyclic synthetic matrix

PPI was expressed as %PPI = (100 [startle response for prepulse/startle response for startle-alone trials] 100)

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