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Zonulin is a protein that increases the permeability of the intestinal barrier and is often involved in the development of autoimmune diseases, including type I diabetes

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Instrumentation All analyses were performed on a 1200 Series HPLC system (Agilent Technologies, Waldbronn, Germany) equipped with a quaternary pump, vacuum degasser, autosampler, module of temperature control, and spectrophotometric detector

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