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A part of the output was directed to a grating-cylindrical lens pulse-shaper to produce a narrowband visible pulse (8-J pulse energy at the sample position, full-width at half-maximum (FWHM) of ~10 cm 1 ), while the other part was used to generate a broadband IR pulse (3-J pulse energy at the sample position, FWHM of ~400 cm 1 ) through an optical parametric amplifier (OPA

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Animals were randomized into 4 groups (16 rats each) and further allocated into 2 cohorts, one for neuronal tracing (n = 6/group) and the other for functional evaluation (n = 10/group)