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Then, samples were centrifugated at 15,000 g for 10 min at 4 C, and the supernatant, containing the extracted D-/L-lactate, was moved into clean tubes

Drug Discov Today 16:600608 McGill M, Jaeschke H (2013) Metabolism and disposition of acetaminophen: recent advances in relation to hepatotoxicity and diagnosis

The reaction was initiated by adding 100 L of 2.5 mM nicotinamide adenine dinucleotide phosphate (NADPH), and after mixing thoroughly, the rate of absorption change at 412 nm was measured spectrophotometrically