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Genetic and epigenetic mechanisms in thyroid autoimmunity

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Thus, we generated pRE-1 and pRE-2 constructs by cloning the 791 bp (739 to +51) and 808 bp (+3493 to +4300) fragments containing these putative p53-binding elements, which were then fused with SV40 promoter, and inserted into the pGL3 luciferase reporter vector