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PLOS ONE 8 , e77023 (2013)

Takahashi et al., 1999)

Efflux function, tissue-specific expression and intracellular trafficking of the Zn transporter ZnT10 indicate roles in adult Zn homeostasis

Inspired by intracellular multi-enzyme cascade reactions, researchers initially attempted to construct artificial cascade systems by encapsulating natural enzymes within porous synthetic materials, including metal-organic frameworks (MOFs), covalent organic frameworks (COFs), and hydrogen-bonded organic frameworks (HOFs) ( 3 O 4 or Au nanoparticles), effectively resolving stability issues ( Figure 3 )

After TMB substrate solution is added, only those wells that contain Mouse MDC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color