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Zhang, S.A

(PubMed) Gao L, Mao Q, Cao J, Wang Y, Zhou X, Fan L

These improvements happen through several ways: It reduces neuronal death by about 65% in brain regions affected by Alzheimer's disease [6] It boosts mitochondrial metabolism in neurons to improve energy production [17] It lowers reactive oxygen species that damage neurons [17] It helps maintain blood-brain barrier integrity [4] NMN works better in older subjects - aged mice respond more favorably to NMN treatment than younger ones [18]

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Protein concentrations in lysates were determined by Protein Assay Dye Reagent (BioRad #5000006), diluted in 2x Laemmli sample buffer (BioRad Cat#1610737) with fresh -mercaptoethanol (BioRad #1610710), and heated at 95C for 5 minutes