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Usually, the body process of skin cell turnover takes 3-4 weeks

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Ultrathin (~70 nm) sections were obtained using Leica EM UC6 Ultramicrotome (Leica, Germany) attached with diamond knife (DiATOME, Switzerland) and double-stained with 1.5% uranyl acetate (15 min at 37 C) followed by 3% lead citrate for 4 min