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As in previous studies [35], we demonstrated that distal lung fibroblasts derived from COPD patients have intrinsic defects in collagen I contraction compared to fibroblasts derived from former smokers without COPD

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Mitochondrial DNA (mtDNA), which encodes 13 essential subunits of the electron transport chain (ETC), is particularly susceptible to oxidative stress, resulting in replication errors, point mutations, and large-scale deletions (Robichaux et al., 2023)

Sex (male versus female) and surgical treatment (HI versus sham) were evaluated as a covariate for CL using the stepwise covariate search, which employed likelihood ratio tests

Trajectory analysis further corroborated these findings (Fig