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This can be achieved either with a solution of a competing ligand or by changing the buffer conditions of the mobile phase, for example to high or low pH, elevated salt concentration, or by applying a chemical denaturant, detergent or chelating agent

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In active acromegaly, many observations show that due to the high GH levels, glucose levels tend to be elevated, which is accompanied by the well-known high insulin levels in untreated acromegaly patients [33,34]