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The absorbance at 360 nm of a reaction mixture containing 20 mM Tris-HCL Ph 7.6, 1 mM EDTA, 10 mM ammonium sulphate, 0.2 mM dithiothreitol (Sigma), 0.2 mM MESG (Berry & associates), 1.25U PNP (Sigma), 4 M small GTPase and 0.5 mM GTP (Cytoskeleton Inc.)