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Cultures were then diluted 1:500 to 1:1,000 in TSB or Seahorse XF DMEM (Agilent) supplemented with 100 mM glucose, 2 mM L-glutamine and 1 mM sodium pyruvate, and dispensed into poly-D-lysine (PDL)-coated Seahorse XF HS miniplates (100 l per well)

The etiology of UC is multifactorial, involving complex interactions among genetic predisposition, immune dysregulation, environmental factors, and gut microbiota alterations [10, 11]

Therefore, it is easy for us to see the progress patients have made in restoring energy levels, sleep, and mood

Single-use supplies, clean prep, and a brief check-in keep things streamlined

Nutrients (2010)