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Experimental procedures for isolated tubules: Tubules were suspended at 1.5-2.0 mg tubule protein/ml in a 95% air/5% CO 2 -gassed medium containing (in mM) 110 NaCl, 2.6 KCl, 25 NaHCO 3 , 2.4 KH 2 PO 4 , 1.25 CaCl 2 , 1.2 MgCl 2 , 1.2 MgSO 4 , 5 glucose, 4 sodium lactate, 0.3 alanine, 5 sodium butyrate, 2 glycine, and 1.0 mg/ml bovine gelatin (75 bloom) (Solution B)

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Rule Out Other Causes If gas persists, consider whether other factors (like diet, lactose intolerance, or gut health issues) may be contributing

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