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Environmental Factors Affecting Biofilm Formation Several environmental factors influence the development and stability of the biofilm: Temperature: Higher temperatures typically enhance microbial activity, resulting in faster biodegradation

Topp MS, van Meerten T, Houot R, Minnema MC, Bouabdallah K, Lugtenburg PJ, et al

This is energetically costly, as the synthesis of each molecule of argininosuccinate requires hydrolysis of adenosine triphosphate (ATP) to adenosine monophosphate (AMP)