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The Phase 2b OPTIONS trial 536 obese subjects, randomized to receive 0.25, 0.5, or 1 mg/day oral AOD-9604 or placebo over 24 weeks failed to demonstrate statistically significant weight loss at any dose tested

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CellTiter-Glo reagent was added to the wells according to the manufacturers instructions and luminescent signal was measured on a SpectraMax M3 microplate reader (Molecular Devices)