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Over time, these proteins are gradually displaced by larger proteins with higher surface affinity, including fibrinogen, immunoglobulins, and apolipoproteins

Additionally, SIN exhibits antidepressant effects, significantly reversing the decrease of NE and serotonin levels in the hippocampus of mice subjected to chronic unpredictable mild stress, with improvements in depression-related symptoms (134)

Front Hum Neurosci 12, 211

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Regardless of the source of the target protein, the initial preparation of a crude sample as a starting point for purification is important and needs to be considered at the same time as thinking about the expression and purification strategies