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Determination of 3-HSD1, 11-HSD1 and 17-HSD1 activity The ovaries were homogenized in ice-cold 10 mM sodium phosphate buffer (pH 7.0) containing 0.25 M sucrose to maintain enzyme stability and tissue integrity

Ng T-P, Chiam P-C, Lee T, Chua H-C, Lim L, Kua E-H

Abbreviations BSO: buthionine sulfoximine GBM: Glioblastomas multiforme GSH: glutathione NAC: N-acetylcysteine NECD: Notch extracellular domain NICD: Notch intracellular domain ROS: reactive oxygen species References Wager M, Fontaine D, Karayan-Tapon L

Appearance of compound K, a major metabolite of ginsenoside Rb1 by intestinal bacteria, in rat plasma after oral administrationmeasurement of compound K by enzyme immunoassay

Its biological activity has been studied comprehensively and can therefore be easily applied in clinical studies (Bouyahya et al., 2023)