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In the Metadynamics simulation, the distance between the two N atoms of the azido group and the C atoms of ethynyl was used as the collective variables to reduce the dimension of the reaction space to calculate the free energy

& Hsu, C.-Y

4AR also decreased gene expression of inflammatory and skin-aging molecules [interleukin (IL-1, IL-6, IL-8), cyclooxygenase-2 (COX-2), tumor necrosis factor receptor super family (TNFRSF)] and the S100 calcium binding proteins A8, A9, suggesting that 4AR 2 has potential for topical treatment and prevention of dermal aging, especially in estrogen-deficient skin

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