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SAHA ameliorates the SMA phenotype in two mouse models for spinal muscular atrophy

finely filtered with a 0.22 m microporous filter membrane, and after the intermediate was qualified, the filtrate was filled in a 15ml vial with 5ml/bottle

doi: 10.1016/j.molmet.2020.01.001 40 DingHGZhangJZhangFZhangSOChenXZLiangWQet al

Added Vitamin B complex: B6 supports immune system

After thawing, the samples were diluted 1:2 with chromatography water followed by sonication (25 min, 20C, SONOREX RK 100, Bandelin, Berlin, Germany) and centrifugation (21,000 x g, 10 min, 4C, Heraeus Fresco 21 Centrifuge, Thermo Scientific, Schwerte, Germany)