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The filtrate was evaporated under a gentle stream of nitrogen (on ice and protected from light and stored at 4 C) and protected from light in an aluminum-covered container, which provided the dry extract as residue

Thats not me denying it definitely doesnt cause an issue in some people, but right now we dont have evidence to support a mechanism in which it would happen

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The mutations can lead to a decrease in the activity and heat resistance of the enzyme, which in turn affects the cycling of folic acid (FA)

Three publicly available databases of biotinidase variants are accessible online: the Leiden Open Variation Database ( ClinVar ( and Biotinidase Deficiency and BTD , hosted by ARUP Laboratories ( Over 200 biotinidase variants are consolidated in the ARUP database, with more than 150 categorized as pathogenic