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As a result, myocardial fibrosis, inflammation, and apoptosis were markedly attenuated, accompanied by improvements in mitochondrial function and calcium homeostasis ( At the in vitro cellular level, numerous studies have employed high-glucose or high-lipid-induced cardiomyocyte models to examine how 4-HNE dysregulation affects cardiomyocyte function, further substantiating its pathogenic role in DCM

doi: 10.1038/s41598-017-03923-2 113 SinghN.JainP.GuptaS.KhuranaJ

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Survival percentages were calculated by dividing the number of surviving cells after exposure to 20 mM H 2 O 2 by the initial cell count prior to the stress treatment