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In addition, GPX 4 neuron-induced knockout (GPX 4 NIKO) mice showed motor neuron degeneration, ALS-like paralytic symptoms and spinal motor neuron death (Evans et al., Multiple studies have demonstrated the treatment of ALS by inhibiting iron death in motor neurons

Fe-sparing responses involve replacement of Fe proteins by alternative enzymes (using either organic cofactors or other metal ions) and a reduction in synthesis of low-priority Fe enzymes to allow Fe use by those pathways most critical for life (Blaby-Haas & Merchant, 2013

Determination of Superoxide Dismutase (SOD) The activity of SOD was done on stomach homogenates using kits provided by Biodiagnostic, Egypt

Excitingly, mice that received Immunocal showed less impairment in their motor and cognitive abilities than controls

Preparations of standard & placebo sample solutions for HPLC analysis Preparation of NAC & Di-NAC standard solutions A stock solution of NAC and Di-NAC standard for method development was prepared by accurately weighed out 50 mg of NAC and Di-NAC transferred into separate 25 ml of volumetric flasks