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To confirm these findings, we depleted ZRANB3 in hTert-immortalized human BJ fibroblasts expressing tamoxifen-inducible HRASV12 (BJ-hTert HRASV12ER-TAM) and in hTert-immortalized human retinal pigment epithelial-1 (RPE1) cells engineered to overexpress cyclin E1 from a doxycycline-regulated promoter 9,37

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